Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice
Identifieur interne : 002B71 ( Main/Exploration ); précédent : 002B70; suivant : 002B72Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice
Auteurs : Nuoyan Zheng [République populaire de Chine] ; Ran Xia [République populaire de Chine] ; Cuiping Yang [République populaire de Chine] ; Bojiao Yin [République populaire de Chine] ; Yin Li [République populaire de Chine] ; Chengguo Duan [République populaire de Chine] ; Liming Liang [République populaire de Chine] ; Huishan Guo [République populaire de Chine] ; Qi Xie [République populaire de Chine]Source :
- Vaccine [ 0264-410X ] ; 2009.
Descripteurs français
- KwdFr :
- Adjuvant Freund (administration et posologie), Adjuvant Freund (pharmacologie), Adjuvants immunologiques (administration et posologie), Adjuvants immunologiques (pharmacologie), Agranulocytes (immunologie), Animaux, Anticorps antiviraux (sang), Extinction de l'expression des gènes, Femelle, Humains, Immunoglobuline G (sang), Injections péritoneales, Interféron gamma (métabolisme), Interleukine-10 (métabolisme), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines nucléocapside (isolement et purification), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Protéines recombinantes (isolement et purification), Rate (immunologie), Souris, Souris de lignée BALB C, Tabac (génétique), Tabac (métabolisme), Tombusvirus (génétique), Vaccins sous-unitaires (génétique), Vaccins sous-unitaires (immunologie), Vaccins sous-unitaires (isolement et purification), Virus du SRAS (génétique), Virus du SRAS (immunologie), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- administration et posologie : Adjuvant Freund, Adjuvants immunologiques.
- génétique : Protéines nucléocapside, Protéines recombinantes, Tabac, Tombusvirus, Vaccins sous-unitaires, Virus du SRAS, Végétaux génétiquement modifiés.
- immunologie : Agranulocytes, Protéines nucléocapside, Protéines recombinantes, Rate, Vaccins sous-unitaires, Virus du SRAS.
- isolement et purification : Protéines nucléocapside, Protéines recombinantes, Vaccins sous-unitaires.
- métabolisme : Interféron gamma, Interleukine-10, Tabac, Végétaux génétiquement modifiés.
- pharmacologie : Adjuvant Freund, Adjuvants immunologiques.
- sang : Anticorps antiviraux, Immunoglobuline G.
- Animaux, Extinction de l'expression des gènes, Femelle, Humains, Injections péritoneales, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (administration & dosage), Adjuvants, Immunologic (pharmacology), Animals, Antibodies, Viral (blood), Female, Freund's Adjuvant (administration & dosage), Freund's Adjuvant (pharmacology), Gene Silencing, Humans, Immunoglobulin G (blood), Injections, Intraperitoneal, Interferon-gamma (metabolism), Interleukin-10 (metabolism), Leukocytes, Mononuclear (immunology), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Nucleocapsid Proteins (isolation & purification), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Recombinant Proteins (isolation & purification), SARS Virus (genetics), SARS Virus (immunology), Spleen (immunology), Tobacco (genetics), Tobacco (metabolism), Tombusvirus (genetics), Vaccines, Subunit (genetics), Vaccines, Subunit (immunology), Vaccines, Subunit (isolation & purification).
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic, Freund's Adjuvant.
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , genetics : Nucleocapsid Proteins, Recombinant Proteins, Vaccines, Subunit.
- chemical , immunology : Nucleocapsid Proteins, Recombinant Proteins, Vaccines, Subunit.
- chemical , isolation & purification : Nucleocapsid Proteins, Recombinant Proteins, Vaccines, Subunit.
- chemical , metabolism : Interferon-gamma, Interleukin-10.
- chemical , pharmacology : Adjuvants, Immunologic, Freund's Adjuvant.
- genetics : Plants, Genetically Modified, SARS Virus, Tobacco, Tombusvirus.
- immunology : Leukocytes, Mononuclear, SARS Virus, Spleen.
- metabolism : Plants, Genetically Modified, Tobacco.
- Animals, Female, Gene Silencing, Humans, Injections, Intraperitoneal, Mice, Mice, Inbred BALB C.
Abstract
Vaccines produced in plant systems are safe and economical; however, the extensive application of plant-based vaccines is mainly hindered by low expression levels of heterologous proteins in plant systems. Here, we demonstrated that the post-transcriptional gene silencing suppressor p19 protein from tomato bushy stunt virus substantially enhanced the transient expression of recombinant SARS-CoV nucleocapsid (rN) protein in
Url:
DOI: 10.1016/j.vaccine.2009.05.073
PubMed: 19523911
PubMed Central: 7115566
Affiliations:
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Le document en format XML
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first="Nuoyan" last="Zheng">Nuoyan Zheng</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Xia, Ran" sort="Xia, Ran" uniqKey="Xia R" first="Ran" last="Xia">Ran Xia</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yang, Cuiping" sort="Yang, Cuiping" uniqKey="Yang C" first="Cuiping" last="Yang">Cuiping Yang</name><affiliation wicri:level="1"><nlm:aff id="aff3">State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yin, Bojiao" sort="Yin, Bojiao" uniqKey="Yin B" first="Bojiao" last="Yin">Bojiao Yin</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3">State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Li, Yin" sort="Li, Yin" uniqKey="Li Y" first="Yin" last="Li">Yin Li</name><affiliation wicri:level="1"><nlm:aff id="aff3">State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Duan, Chengguo" sort="Duan, Chengguo" uniqKey="Duan C" first="Chengguo" last="Duan">Chengguo Duan</name><affiliation wicri:level="1"><nlm:aff id="aff2">State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liang, Liming" sort="Liang, Liming" uniqKey="Liang L" first="Liming" last="Liang">Liming Liang</name><affiliation wicri:level="1"><nlm:aff id="aff3">State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Biocontrol, Sun Yat-sen (Zhongshan) University, 135 Xingang Road W, Guangzhou 510275</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Guo, Huishan" sort="Guo, Huishan" uniqKey="Guo H" first="Huishan" last="Guo">Huishan Guo</name><affiliation wicri:level="1"><nlm:aff id="aff2">State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xie, Qi" sort="Xie, Qi" uniqKey="Xie Q" first="Qi" last="Xie">Qi Xie</name><affiliation wicri:level="1"><nlm:aff id="aff1">State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Datun Road, Beijing 100101</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (administration & dosage)</term><term>Adjuvants, Immunologic (pharmacology)</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Female</term><term>Freund's Adjuvant (administration & dosage)</term><term>Freund's Adjuvant (pharmacology)</term><term>Gene Silencing</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Injections, Intraperitoneal</term><term>Interferon-gamma (metabolism)</term><term>Interleukin-10 (metabolism)</term><term>Leukocytes, Mononuclear (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Nucleocapsid Proteins (isolation & purification)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Recombinant Proteins (isolation & purification)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Spleen (immunology)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Tombusvirus (genetics)</term><term>Vaccines, Subunit (genetics)</term><term>Vaccines, Subunit (immunology)</term><term>Vaccines, Subunit (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvant Freund (administration et posologie)</term><term>Adjuvant Freund (pharmacologie)</term><term>Adjuvants immunologiques (administration et posologie)</term><term>Adjuvants immunologiques (pharmacologie)</term><term>Agranulocytes (immunologie)</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Extinction de l'expression des gènes</term><term>Femelle</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Injections péritoneales</term><term>Interféron gamma (métabolisme)</term><term>Interleukine-10 (métabolisme)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines nucléocapside (isolement et purification)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Protéines recombinantes (isolement et purification)</term><term>Rate (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Tombusvirus (génétique)</term><term>Vaccins sous-unitaires (génétique)</term><term>Vaccins sous-unitaires (immunologie)</term><term>Vaccins sous-unitaires (isolement et purification)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Freund's Adjuvant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term><term>Vaccines, Subunit</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term><term>Vaccines, Subunit</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term><term>Vaccines, Subunit</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferon-gamma</term><term>Interleukin-10</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Adjuvants, Immunologic</term><term>Freund's Adjuvant</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvant Freund</term><term>Adjuvants immunologiques</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>SARS Virus</term><term>Tobacco</term><term>Tombusvirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Tabac</term><term>Tombusvirus</term><term>Vaccins sous-unitaires</term><term>Virus du SRAS</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Agranulocytes</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Rate</term><term>Vaccins sous-unitaires</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Leukocytes, Mononuclear</term><term>SARS Virus</term><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Vaccins sous-unitaires</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Interféron gamma</term><term>Interleukine-10</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Adjuvant Freund</term><term>Adjuvants immunologiques</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Gene Silencing</term><term>Humans</term><term>Injections, Intraperitoneal</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Extinction de l'expression des gènes</term><term>Femelle</term><term>Humains</term><term>Injections péritoneales</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Vaccines produced in plant systems are safe and economical; however, the extensive application of plant-based vaccines is mainly hindered by low expression levels of heterologous proteins in plant systems. Here, we demonstrated that the post-transcriptional gene silencing suppressor p19 protein from tomato bushy stunt virus substantially enhanced the transient expression of recombinant SARS-CoV nucleocapsid (rN) protein in <italic>Nicotiana benthamiana</italic>. The rN protein in the agrobacteria-infiltrated plant leaf accumulated up to a concentration of 79 μg per g fresh leaf weight at 3 days post infiltration. BALB/c mice were intraperitoneally vaccinated with pre-treated plant extract emulsified in Freund's adjuvant. The rN protein-specific IgG in the mouse sera attained a titer about 1:1,800 following three doses of immunization, which suggested effective B-cell maturation and differentiation in mice. Antibodies of the subclasses IgG1 and IgG2a were abundantly present in the mouse sera. During vaccination of rN protein, the expression of IFN-γ and IL-10 was evidently up-regulated in splenocytes at different time points, while the expression of IL-2 and IL-4 was not. 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Zhang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zheng, Nuoyan" sort="Zheng, Nuoyan" uniqKey="Zheng N" first="Nuoyan" last="Zheng">Nuoyan Zheng</name></noRegion><name sortKey="Duan, Chengguo" sort="Duan, Chengguo" uniqKey="Duan C" first="Chengguo" last="Duan">Chengguo Duan</name><name sortKey="Guo, Huishan" sort="Guo, Huishan" uniqKey="Guo H" first="Huishan" last="Guo">Huishan Guo</name><name sortKey="Li, Yin" sort="Li, Yin" uniqKey="Li Y" first="Yin" last="Li">Yin Li</name><name sortKey="Liang, Liming" sort="Liang, Liming" uniqKey="Liang L" first="Liming" last="Liang">Liming Liang</name><name sortKey="Xia, Ran" sort="Xia, Ran" uniqKey="Xia R" first="Ran" last="Xia">Ran Xia</name><name sortKey="Xie, Qi" sort="Xie, Qi" uniqKey="Xie Q" first="Qi" last="Xie">Qi Xie</name><name sortKey="Yang, Cuiping" sort="Yang, Cuiping" uniqKey="Yang C" first="Cuiping" last="Yang">Cuiping Yang</name><name sortKey="Yin, Bojiao" sort="Yin, Bojiao" uniqKey="Yin B" first="Bojiao" last="Yin">Bojiao Yin</name><name sortKey="Yin, Bojiao" sort="Yin, Bojiao" uniqKey="Yin B" first="Bojiao" last="Yin">Bojiao Yin</name><name sortKey="Zheng, Nuoyan" sort="Zheng, Nuoyan" uniqKey="Zheng N" first="Nuoyan" last="Zheng">Nuoyan Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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